Amino acid analysis of the modified enzyme revealed that the N-terminal amino group was modified, and that the C-terminal amino acid was truncated by 30 carboxyamino acids.
Methylation of the C-terminal amino group of the enzyme and proteolytic cleavage of the N-terminal amino acid residue resulted in the modification of the protein to a form of lower molecular weight, identical to that of the inactivated enzyme. The major changes observed in the EDTA-modified enzyme were a reduction in thermal stability and a decrease in gel mobility, with the conformation of the protein remaining unchanged.
However, when the molar excess of EDTA exceeded the stoichiometric amount of bound metal ion, the EDTA-modified enzyme conformation differed significantly from that of the native enzyme. When the amount of EDTA added was in the molar range that was associated with the inactivation, protein conformations in native and EDTA-modified enzyme were indistinguishable. Molecular sieve chromatography of the enzyme-EDTA complex resulted in separation of three protein species with the same mobility as the native enzyme, two EDTA-inactivated forms and one EDTA-modified form. The EDTA-modified protein had a lower activity than that of the native enzyme. The inactivation rate followed first-order kinetics with respect to EDTA concentration, indicating that one enzyme subunit is modified by two moles of the chelator. Phosphofructokinase from rabbit muscle was inactivated by EDTA. Norton Ghost creates a backup of all your files, contacts and .ġ) to create snapshots of your hard drive and keep them safe forever.Structure of the N-terminal domain of the central subunit of rabbit muscle phosphofructokinase. Symantec brings you Norton Ghost 8.0 Free and Easy-to-use Software.Ģ) to back up your important data, which can be used for installing the Norton Ghost 3.8. It is a disk-imaging tool and also can be used to back up a Windows PC.Ĩ/10 (7 votes) – Download Norton Ghost 8.0. You can get Norton Ghost 8.0 free from Symantec’s Download Center. Click on this link to get free norton ghost 8.0 download . Symantec norton ghost 8.0 free download – . How To Backup And Restore Norton Ghost 2003 Norton Ghost can make disk image or specific files and folders, you decide what will include your backup. Select Version of Norton Ghost to Download for FREE! Official Website: Company: Symantec Corporation Recently added version. It is well-suited for placement on bootable media, such as BartPE′s bootable CD. Norton Ghost is a good backup program for a user who is looking for an easy-to-use, reliable, and efficient way to backup their data, as well as a way to create bootable media.
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